Pc10 Manual New: Narishige

Single-stage pull (for large tips) or dual-stage pull (for patches and microinjection tips).

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The secondary heating phase triggers automatically, followed immediately by the final drop that breaks the glass into two identical pipettes. Carefully remove both pipettes from the clamps. 5. Parameter Optimization and Troubleshooting Single-stage pull (for large tips) or dual-stage pull

Increase the Magnet dial value. A faster pull rate snaps the glass at a thinner cross-section. Let the unit cool

If the coil accumulates debris, run a high-heat cycle without any glass installed for 2–3 seconds to burn off residues. Troubleshooting Common Issues Probable Cause Corrective Action Glass does not break / melts completely Heater value is set too low or too high. Readjust dials incrementally by 2–3 units. Asymmetrical pipette tips Heating coil is uncentered or distorted. Let the unit cool, then manually center the coil. Inconsistent pipette resistance Drafts in the room or friction on the shaft. Close the draft shield; clean the vertical shaft. Unit will not turn on Blown fuse or faulty power connection. Check the rear fuse drawer and replace if necessary. 6. Conclusion

Controls the electrical current sent to the platinum coil during the first and second pull stages. Higher numbers increase the temperature.

Carefully remove the upper and lower pipettes vertically to avoid snapping the fragile tips.